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Jia Liu from the Laboratory of ADC Chemistry published a paper on ¡°the Journal of Visualized Experiments (JoVE)¡± in collaboration with the Scripps Research Institute


Date£º13 April 2015

Research Associate Professor Jia Liu from the Laboratory of ADC Chemistry recently published a paper in collaboration with the Scripps Research Institute. This paper, entitled ¡°Direct Protein Delivery to Mammalian Cells Using Cell-permeable Cys2-His2 Zinc-finger Domains¡±, was published in Journal of Visualized Experiments (JoVE) (doi:10.3791/52814).

Due to their modularity and ability to be reprogrammed to recognize a wide range of DNA sequences, Cys2-His2 zinc-finger DNA-binding domains have emerged as useful tools for targeted genome engineering. Like many other DNA-binding proteins, zinc-fingers also possess the innate ability to cross cell membranes. Dr. Liu, teamed up with Dr. Gaj from The Scripps Research Institute, recently demonstrated that this intrinsic cell-permeability could be leveraged for intracellular protein delivery. Genetic fusion of zinc-finger motifs leads to efficient transport of protein and enzyme cargo into a broad range of mammalian cell types. Unlike other protein transduction technologies, delivery via zinc-finger domains does not inhibit enzyme activity and leads to high levels of cytosolic delivery. Here a detailed step-by-step protocol is presented for the implementation of zinc-finger technology for protein delivery into mammalian cells. Key steps for achieving high levels of intracellular zinc-finger-mediated delivery are highlighted and strategies for maximizing the performance of this system are discussed.

¡°We have seen efficient delivery of a variety of proteins under cell culture condition using this ZiF platform. Now we are exploring its application in animals and, hopefully, can develop some ZiF product of commercial interest.¡± Jia Liu said.



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