特聘教授 课题组长
所在学院 免疫化学研究所
生命科学与技术学院
研究方向 蛋白质机器与新药
联系方式 raozh@@shanghaitech.edu.cn
raozh@@mail.tsinghua.edu.cn
备注 中国科学院院士
主要研究内容
饶子和教授主要利用X-ray和Cro-EM等现代生物物理技术手段，研究人类新发、再发传染病病原体的蛋白质机器的结构与功能，以及基于结构的创新药物开发。他的研究重点关注结核分枝杆菌的关键药物靶点，包括细胞壁合成、呼吸链和转录、复制相关的超级分子机器。同时，饶子和教授长期从事SARS病毒、流感病毒、艾滋病病毒、肝炎病毒、疱疹病毒、小RNA病毒和黄病毒等重要病原体在宿主入侵、复制和组装过程中起关键作用的分子机器的研究，并针对性的开发新型抗病毒药物和疫苗。
代表性论文
2020
383. Structural Basis for RNA Replication by the SARS-CoV-2 Polymerase. Wang Q, Wu J, Wang H, Gao Y, Liu Q, Mu A, Ji W, Yan L, Zhu Y, Zhu C, Fang X, Yang X, Huang Y, Gao H, Liu F, Ge J, Sun Q, Yang X, Xu W, Liu Z, Yang H, Lou Z, Jiang B, Guddat LW, Gong P,Rao Z. Cell. 2020 May 22:S0092-8674(20)30629-2.
382. Crystal structure of the African swine fever virus structural protein p35 reveals its role for core shell assembly. Li G, Fu D, Zhang G, Zhao D, Li M, Geng X, Sun D, Wang Y, Chen C, Jiao P, Cao L, Guo Y, Rao Z. Protein Cell. 2020 Jun 9.
381. Structural Basis for the Inhibition of Mycobacterial MmpL3 by NITD-349 and SPIRO. Yang X, Hu T, Yang X, Xu W, Yang H, Guddat LW, Zhang B, Rao Z.J Mol Biol.2020 Jun 5:S0022-2836(20)30368-5.
380. Structure of African swine fever virus p15 reveals its dual role for membrane-association and DNA binding. Fu D, Zhao D, Zhang W, Zhang G, Li M, Zhang Z, Wang Y, Sun D, Jiao P, Chen C, Guo Y,Rao Z.Protein Cell. 2020 May 25.
379. Structural basis for the inhibition of SARS-CoV-2 main protease by antineoplastic drug carmofur. Jin Z, Zhao Y, Sun Y, Zhang B, Wang H, Wu Y, Zhu Y, Zhu C, Hu T, Du X, Duan Y, Yu J, Yang X, Yang X, Yang K, Liu X, Guddat LW, Xiao G, Zhang L, Yang H, Rao Z. Nat Struct Mol Biol. 2020 Jun;27(6):529-532.
378. Structural insights into G domain dimerization and pathogenic mutation of OPA1. Yu C, Zhao J, Yan L, Qi Y, Guo X, Lou Z, Hu J, Rao Z. J Cell Biol. 2020 Jul 6;219(7):e201907098.
377. Cryo-EM snapshots of mycobacterial arabinosyltransferase complex EmbB2-AcpM2. Zhang L, Zhao Y, Gao R, Li J, Yang X, Gao Y, Zhao W, Gurcha SS, Veerapen N, Batt SM, Besra KK, Xu W, Bi L, Zhang X, Guddat LW, Yang H, Wang Q, Besra GS, Rao Z. Protein Cell. 2020 May 3.
376. Structure of severe fever with thrombocytopenia syndrome virus L protein elucidates the mechanisms of viral transcription initiation. Wang P, Liu L, Liu A, Yan L, He Y, Shen S, Hu M, Guo Y, Liu H, Liu C, Lu Y, Wang P, Deng F, Rao Z, Lou Z. Nat Microbiol. 2020 Jun;5(6):864-871.
375. Architecture of the herpesvirus genome-packaging complex and implications for DNA translocation. Yang Y, Yang P, Wang N, Chen Z, Su D, Zhou ZH, Rao Z, Wang X. Protein Cell. 2020 May;11(5):339-351.
374. Structures of cell wall arabinosyltransferases with the anti-tuberculosis drug ethambutol. Zhang L, Zhao Y, Gao Y, Wu L, Gao R, Zhang Q, Wang Y, Wu C, Wu F, Gurcha SS, Veerapen N, Batt SM, Zhao W, Qin L, Yang X, Wang M, Zhu Y, Zhang B, Bi L, Zhang X, Yang H, Guddat LW, Xu W, Wang Q, Li J, Besra GS, Rao Z. Science. 2020 Jun 12;368(6496):1211-1219.
373. Structure-based design of antiviral drug candidates targeting the SARS-CoV-2 main protease. Dai W, Zhang B, Su H, Li J, Zhao Y, Xie X, Jin Z, Liu F, Li C, Li Y, Bai F, Wang H, Cheng X, Cen X, Hu S, Yang X, Wang J, Liu X, Xiao G, Jiang H, Rao Z, Zhang LK, Xu Y, Yang H, Liu H. Science. 2020 Apr 22:eabb4489.
372. Structures of the portal vertex reveal essential protein-protein interactions for Herpesvirus assembly and maturation. Wang N, Chen W, Zhu L, Zhu D, Feng R, Wang J, Zhu B, Zhang X, Chen X, Liu X, Yan R, Ni D, Zhou GG, Liu H, Rao Z, Wang X. Protein Cell. 2020 May;11(5):366-373.
371. Structure of the RNA-dependent RNA polymerase from COVID-19 virus.
Gao Y, Yan L, Huang Y, Liu F, Zhao Y, Cao L, Wang T, Sun Q, Ming Z, Zhang L, Ge J, Zheng L, Zhang Y, Wang H, Zhu Y, Zhu C, Hu T, Hua T, Zhang B, Yang X, Li J, Yang H, Liu Z, Xu W, Guddat LW, Wang Q, Lou Z, Rao Z. Science. 2020 May 15;368(6492):779-782.
370. Structure of Mpro from SARS-CoV-2 and discovery of its inhibitors. Jin Z, Du X, Xu Y, Deng Y, Liu M, Zhao Y, Zhang B, Li X, Zhang L, Peng C, Duan Y, Yu J, Wang L, Yang K, Liu F, Jiang R, Yang X, You T, Liu X, Yang X, Bai F, Liu H, Liu X, Guddat LW, Xu W, Xiao G, Qin C, Shi Z, Jiang H, Rao Z, Yang H. Nature. 2020 Jun;582(7811):289-293.
369. Structures of Mycobacterium tuberculosis Penicillin-Binding Protein 3 in Complex with Five β-Lactam Antibiotics Reveal Mechanism of Inactivation. Lu Z, Wang H, Zhang A, Liu X, Zhou W, Yang C, Guddat L, Yang H, Schofield CJ, Rao Z. Mol Pharmacol. 2020 Apr;97(4):287-294.
368. Crystal Structure of African Swine Fever Virus pS273R Protease and Implications for Inhibitor Design. Li G, Liu X, Yang M, Zhang G, Wang Z, Guo K, Gao Y, Jiao P, Sun J, Chen C, Wang H, Deng W, Xiao H, Li S, Wu H, Wang Y, Cao L, Jia Z, Shang L, Yang C, Guo Y, Rao Z. J Virol. 2020 May 4;94(10):e02125-19.
367. Structural analysis of a trimeric assembly of the mitochondrial dynamin-like GTPase Mgm1. Yan L, Qi Y, Ricketson D, Li L, Subramanian K, Zhao J, Yu C, Wu L, Sarsam R, Wong M, Lou Z, Rao Z, Nunnari J, Hu J. Proc Natl Acad Sci U S A. 2020 Feb 25;117(8):4061-4070.
366. Identification of Interferon Receptor IFNAR2 As a Novel HCV Entry Factor by Using Chemical Probes. Qing J, Wu M, Luo R, Chen J, Cao L, Zeng D, Shang L, Nong J, Wu Q, Ding BS, Chen X, Rao Z, Liu L, Lou Z. ACS Chem Biol. 2020 May 15;15(5):1232-1241.
365. Host-mediated ubiquitination of a mycobacterial protein suppresses immunity. Wang L, Wu J, Li J, Yang H, Tang T, Liang H, Zuo M, Wang J, Liu H, Liu F, Chen J, Liu Z, Wang Y, Peng C, Wu X, Zheng R, Huang X, Ran Y, Rao Z, Ge B. Nature. 2020 Jan;577(7792):682-688. doi: 10.1038/s41586-019-1915-7.
364. Design, Synthesis, and Evaluation of Novel Enterovirus 71 Inhibitors as Therapeutic Drug Leads for the Treatment of Human Hand, Foot, and Mouth Disease. Zhang M, Wang Y, He W, Sun Y, Guo Y, Zhong W, Gao Q, Liao M, Wang X, Cai Y, Guo Y, Rao Z. J Med Chem. 2020 Feb 13;63(3):1233-1244.
363. Hand-foot-and-mouth disease virus receptor KREMEN1 binds the canyon of Coxsackie Virus A10. Zhao Y, Zhou D, Ni T, Karia D, Kotecha A, Wang X, Rao Z, Jones EY, Fry EE, Ren J, Stuart DI. Nat Commun. 2020 Jan 7;11(1):38.
362. Structural insights into substrate recognition by the type VII secretion system. Wang S, Zhou K, Yang X, Zhang B, Zhao Y, Xiao Y, Yang X, Yang H, Guddat LW, Li J, Rao Z. Version 2. Protein Cell. 2020 Feb;11(2):124-137.
361. Structural Insight into African Swine Fever Virus dUTPase Reveals a Novel Folding Pattern in the dUTPase Family. Li G, Wang C, Yang M, Cao L, Fu D, Liu X, Sun D, Chen C, Wang Y, Jia Z, Yang C, Guo Y, Rao Z. J Virol. 2020 Jan 31;94(4):e01698-19.
2019
360. Structural basis for enzymatic photocatalysis in chlorophyll biosynthesis. Zhang S, Heyes DJ, Feng L, Sun W, Johannissen LO, Liu H, Levy CW, Li X, Yang J, Yu X, Lin M, Hardman SJO, Hoeven R, Sakuma M, Hay S, Leys D, Rao Z, Zhou A, Cheng Q, Scrutton NS. Nature. 2019 Oct;574(7780):722-725. doi: 10.1038/s41586-019-1685-2. Epub 2019 Oct 23.
359. Architecture of African swine fever virus and implications for viral assembly. Wang N, Zhao D, Wang J, Zhang Y, Wang M, Gao Y, Li F, Wang J, Bu Z, Rao Z, Wang X. Science. 2019 Nov 1;366(6465):640-644. doi: 10.1126/science.aaz1439. Epub 2019 Oct 17.
358. Mycobacterial dynamin-like protein IniA mediates membrane fission.Wang M, Guo X, Yang X, Zhang B, Ren J, Liu A, Ran Y, Yan B, Chen F, Guddat LW, Hu J, Li J, Rao Z. Nat Commun. 2019 Aug 29;10(1):3906. doi: 10.1038/s41467-019-11860-z.
357. Divergent engagements between adeno-associated viruses with their cellular receptor AAVR. Zhang R, Xu G, Cao L, Sun Z, He Y, Cui M, Sun Y, Li S, Li H, Qin L, Hu M, Yuan Z, Rao Z, Ding W, Rao Z, Lou Z. Nat Commun. 2019 Aug 21;10(1):3760. doi: 10.1038/s41467-019-11668-x.
356. Delicate structural coordination of the Severe Acute Respiratory Syndrome coronavirus Nsp13 upon ATP hydrolysis. Jia Z, Yan L, Ren Z, Wu L, Wang J, Guo J, Zheng L, Ming Z, Zhang L, Lou Z, Rao Z.Nucleic Acids Res. 2019 May 27; pii: gkz409. doi: 10.1093/nar/gkz409.
355. Structural basis for neutralization of hepatitis A virus informs a rational design of highly potent inhibitors. Cao L, Liu P, Yang P, Gao Q, Li H, Sun Y, Zhu L, Lin J, Su D*, Rao Z*, Wang X*. PLoS Biol. 2019 Apr 30;17(4):e3000229. doi: 10.1371/journal.pbio.3000229. eCollection 2019 Apr. PMID: 31039149 Free Article
354.Purification and characterization of the extracellular region of human receptor tyrosine kinase like orphan receptor 2 (ROR2). Li Y, Han X, Xu W, Rao Z, Li X*. Protein Expr Purif. 2019 Jun;158:74-80. doi: 10.1016/j.pep.2019.02.015.
353.Structural analysis of molybdopterin synthases from two mycobacterial pathogens. Wang H, Chen X, Zhang W, Zhou W, Liu X, Rao Z. Biochem Biophys Res Commun. 2019 Mar 26;511(1):21-27. doi: 10.1016/j.bbrc.2019.02.024.
352. Adeno-associated virus 2 bound to its cellular receptor AAVR. Zhang R, Cao L, Cui M, Sun Z, Hu M, Zhang R, Stuart W, Zhao X, Yang Z, Li X, Sun Y, Li S, Ding W*, Lou Z*, Rao Z. Nat Microbiol. 2019 Apr;4(4):675-682. doi: 10.1038/s41564-018-0356-7.
351.The crystal structure of the phosphotriesterase from M. tuberculosis, another member of phosphotriesterase-like lactonase family. Zhang L, Wang H, Liu X, Zhou W, Rao Z. Biochem Biophys Res Commun. 2019 Mar 5;510(2):224-229.
350.Study of the whole genome, methylome and transcriptome of Cordyceps militaris. Chen Y, Wu Y, Liu L, Feng J, Zhang T, Qin S, Zhao X, Wang C, Li D, Han W, Shao M, Zhao P, Xue J, Liu X, Li H, Zhao E, Zhao W, Guo X, Jin Y, Cao Y, Cui L, Zhou Z, Xia Q, Rao Z, Zhang Y. Sci Rep. 2019 Jan 29;9(1):898.
349.Crystal Structures of Membrane Transporter MmpL3, an Anti-TB Drug Target. Zhang B, Li J*, Yang X, Wu L, Zhang J, Yang Y, Zhao Y, Zhang L, Yang X, Yang X, Cheng X, Liu Z, Jiang B, Jiang H, Guddat LW, Yang H*, Rao Z*. Cell. 2019 Jan 24;176(3):636-648.e13
348.Crystal structure and biochemical study on argininosuccinate lyase from Mycobacterium tuberculosis. Chen X, Chen J, Zhang W, Wang H, Liu X, Zhou W, Yang H, Rao Z. Biochem Biophys Res Commun. 2019 Jan 18. pii: S0006-291X(19)30075-0.
347.Double Lock of a Human Neutralizing and Protective Monoclonal Antibody Targeting the Yellow Fever Virus Envelope. Lu X, Xiao H, Li S, Pang X, Song J, Liu S, Cheng H, Li Y, Wang X, Huang C, Guo T, Ter Meulen J, Daffis S, Yan J, Dai L, Rao Z, Klenk HD, Qi J, Shi Y, Gao GF. Cell Rep. 2019 Jan 8;26(2):438-446.e5.
