SIAIS Analytical Chemistry Platform

Analytical platform is a core facility funded by SIAIS-Shanghaitech University. The goal of the platform is to provide biomolecules characterization services to scientists at Shanghaitech University and external clients globally.

The platform is equipped with state-of-the-art facility including two Orbitrap Fusion MS, one QTOF MS, one 500M NMR, one two-D UPLC, one GCMS, etc. A wide range of software tools including Proteome Discover, Maxquant, MASCOT, SCAFFOLD, HDExaminer, PEAKS, Biopharma, and SimGlycan are also available for the following Database searching and data visualization. The platform staff work closely with SIAIS bioinformatics team to provide statistical solution to our clients.

Major instruments
nLC-Orbitrap Fusion MS (Thermo Scientific)
The instrument is equipped with nano UPLC and Easyspary source to ensure the best HPLC separation resolution. The instrument is used to identify and quantify a variety type of samples such as single gel band sample and whole cell lysate sample.
HDX-Orbitrap Fusion (Thermo Scientific)
The instrument is coupled with Leap robot sample preparation system. The instrument is used to identify the binding site of a drug or antibody (epitope mapping) on its target protein by using Hydrogen/Deuterium Exchange technique.
Hybrid Quadrupole-Time-of-Flight MS (Sciex 4600)
The instrument is coupled with SHIMADZU UPLC system to improve peak resolution and throughput. The instrument is mainly utilized to measure exact mass of synthetic small molecules, oligonucleotides, and other biomolecules.
500M NMR spectrometer (Bruker)
Small molecule structure characterization
MALDI-TOF/TOF-MS (Sciex, 5800)
High throughput MS determination
2-D UPLC system (Agilent)