Research Associate Professor, Director of Protein & Gene Platform
Laboratory: Lab of Structure Biochemistry
Research Interest: Elucidating the molecular mechanisms of multiprotein complexes involved in transcriptional regulation by combining the MultiBac technology, X-ray crystallography, cryo-EM, and other powerful methods. Developing advanced eukaryotic expression technologies for multiprotein biologics.
Email: nieyan@@shanghaitech.edu.cn
Biography:
2015.11-present Co-Principal Investigator, Research Associate Professor (double as the Director of Protein & Gene Platform since July 2016), Shanghai Institute for Advanced Immunochemical Studies (SIAIS), ShanghaiTech University, Shanghai, China.
2013.11-2015.08 Postdoctoral Fellow, MPI of Molecular Physiology (Prof. Dr. Stefan Raunser), Dortmund, Germany.
2013.01-10 Postdoctoral Fellow, EMBL Grenoble (Prof. Dr. Imre Berger), Grenoble, France.
2012 Ph.D. in Molecular Biology, EMBL Grenoble, Grenoble, France.
2008 M.Sc. in Physics (specialized in Biophysics), Royal Institute of Technology (KTH), Stockholm, Sweden.
2005 B.Sc. in Physics, Fudan University, Shanghai, China.
Selected Grants, Awards, & Honors:
2017.11 Gold Medal, Best Hardware and other four Nominations (as one of the advisors of the Shanghaitech team), iGEM 2017（2017年国际基因工程机器大赛）, Boston, USA.
2016.10 Gold Medal, iGEM 2016（2016年国际基因工程机器大赛）(as an instructor of the ShanghaitechChina team), Boston, USA.
2016.08 Young Scientists Fund of the National Natural Science Foundation of China（国家自然科学基金青年科学基金项目） (Grant No.: 31600602; 2017.01-2019.01).
2010.05 2009 Chinese Government Award for Outstanding Self-Financed Students Abroad / 2009年度国家优秀自费留学生奖学金（法国领区）.
2009-2011 Boehringer Ingelheim Fonds PhD Fellowship.
Representative publications:
[1] Nie, Y., Chaillet, M., Becke, C., Haffke, M., Pelosse, M., Fitzgerald, D., Collinson, I., Schaffitzel, C. & Berger, I. ACEMBL Tool-Kits for High-Throughput Multigene Delivery and Expression in Prokaryotic and Eukaryotic Hosts. Adv. Exp. Med. Biol. 896, 27–42 (2016).
[2] Frisch, H., Nie, Y., Raunser, S. & Besenius, P. pH-Regulated Selectivity in Supramolecular Polymerizations: Switching between Co- and Homopolymers. Chem. Eur. J.21, 3304–3309 (2015).
[3] Nie, Y.,Bellon-Echeverria, I., Trowitzsch, S., Bieniossek, C. & Berger, I. Multiprotein complex production in insect cells by using polyproteins. Methods Mol. Biol. 1091, 131–141 (2014).
[4] Nie, Y., Viola, C., Bieniossek, C., Trowitzsch, S., Vijay-Achandran, L.S., Chaillet, M., Garzoni, F. & Berger, I. Getting a Grip on Complexes. Curr. Genomics10, 558–572 (2009).
[5] Bieniossek, C.*, Nie, Y.*, Frey, D., Olieric, N., Schaffitzel, C., Collinson, I., Romier, C., Berger, P., Richmond, T.J., Steinmetz, M.O. & Berger, I. Automated unrestricted multigene recombineering for multiprotein complex production. Nat. Methods6, 447–450 (2009).
*Contributed equally
All publicationlist: https://www.researchgate.net/profile/Yan_Nie3
