课题组长, 研究员, 助理教授
Principal Investigator, Tenure-track Assistant Professor
Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University
Email: yangbei@shanghaitech.edu.cn
Tel: 021-20685590
Laboratory: Lab of Pathogenic Biology
Research Interest
Research in our lab focuses on several high-risk infectious diseases and their causative microbes, including respiratory viruses, HIV-1 and plasmodium. We are particularly interested in:
1) identifying novel sites of therapeutic and preventive vulnerabilities in these microbial pathogens;
2) rational screen and development of potential antivirals against their infections;
3) structure-guided engineering & functional reshaping of host factors for the treatment or prevention of these high-risk infectious diseases.
Expertise
Integrative Structural Biology, Structural Proteomics
Biography
2004 B.S. National Talents Training Program, College of Life Sciences, Wuhan University
2010 Ph.D. of Biochemistry and Molecular Biology, Institute of Biochemistry and Cell Biology, Chinese Academy of Science
2010-2013 Visiting Fellow, National Institutes of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, U.S.
2013-2015 Postdoctoral Scholar, Department of Molecular and Cell Biology, University of California, Berkeley, U.S.
2015.11-2020.09 Co-PI, Research Associate Professor, Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China
2020.09-present Principle investigator & Tenure-track assistant professor, Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China
Selected Awards and Honors
2017 Young Scholar, Natural Science Foundation of China
2016 Pujiang Scholar, Science and Technology Commission of Shanghai Municipality
Selected Publications: (* co-first author, # correspondence author)
1. Xiang J*, Su J*, Lan Q*, Zhao W, Zhou Y, Xu Y, Niu J, Xia S, Qi Q, Sidhu S, Lu L#, Miersch S#, Yang B#. (2022) Antigenic mapping reveals sites of vulnerability on α-HCoV spike protein. Communications Biology, 5(1):1179
2. Jia X*, Li Y*, Wang T, Bi L, Guo L, Chen Z, Zhang X, Ye S, Chen J, Yang B, Sun B. (2022) Discrete RNA-DNA hybrid cleavage by the EXD2 exonuclease pinpoints two rate-limiting steps. EMBO Journal, 3:e111703
3. Li X*, Zhou L*, Gao BQ*, Li G, Wang X, Wang Y, Wei J, Han W, Wang Z, Li J, Gao R, Zhu J, Xu W, Wu J, Yang B, Sun X#, Yang L#, Chen J#. (2022) Highly efficient prime editing by introducing same-sense mutations in pegRNA or stabilizing its structure. Nature Communications, 13(1):1669
4. Wang LJ*, Xue W*, Zhang HX*, Gao RZ*, Qiu HY*, Wei J, Zhou LN, Lei YN, Wu XC, Li X, Liu CF, Wu J, Chen QB, Ma HH, Huang XX, Cai C, Zhang Y, Yang B#, Yin H#, Yang L#, Chen J#. (2021) Eliminating genome-wide and transcriptome-wide off-target mutations by base editor. Nature Cell Biology, DOI:10.1038/S41556-021-00671-4
5. Shi X*, Wan Y*, Wang N*, Xiang J*, Wang T, Yang X, Wang J, Dong X, Dong L, Yan L, Li Y, Liu L, Hou S, Zhong Z, Wilson IA, Yang B, Yang G#, Lerner RA#.(2021) Selection of a picomolar antibody that targets CXCR2-mediated neutrophil activation and alleviates EAE symptoms. Nature Communications,12(1):2547
6. Meng B, Lan KK, Xie J, Lerner RA, Wilson IA#, Yang B#. (2020) Inhibitory antibodies identify novel sites of therapeutic vulnerability in rhinovirus and other Enteroviruses. Proceedings of the National Academy of Sciences, 117 (24): 13499-13508
7. Wang X*, Ding C*, Yu W*, Wang Y*, He S*, Yang B*, Xiong YC, Wei J, Li J, Liang J, Lu Z, Zhu W, Wu J, Zhou Z, Huang X, Liu Z#, Yang L#, Chen J#. (2020) Cas12a Base Editors Induce Efficient and Specific Editing with Low DNA Damage Response. Cell Report, 31(9):107723
8. Cui YR*, Wang SJ*, Chen J, Li J, Chen W, Wang S, Meng B, Zhu W, Zhang Z, Yang B, Jiang B, Yang G, Ma P#., Liu J#. (2020) Allosteric inhibition of CRISPR-Cas9 by bacteriophage-derived peptides. Genome Biology, 21(1):51
9. Yang L#, Yang B#, Chen J#. (2019) One Prime for All Editing. Cell, 179(7):1448-1450
10. Xu JC*, Cui KJ*, Shen LQ, Shi J, Li LT, You LL, Fang CL, Zhao GP#, Feng Y#, Yang B#, Zhang Y#. (2019) Crl activates transcription by stabilizing active conformation of the master stress transcription initiation factor. eLife, 8: e50928
11. Zhao Q*, Ren C*, Liu L, Chen J, Shao Y, Sun N, Sun R, Kong Y, Ding X, Zhang X, Xu Y, Yang B, Yin Q#, Yang X#, Jiang B#. (2019) Discovery of SIAIS178 as an Effective BCR-ABL Degrader by Recruiting Von Hippel-Lindau (VHL) E3 Ubiquitin Ligase. Journal of Medicinal Chemistry, 62(20):9281-9298
12. Wang Y*, Gao R*, Wu J, Xiong YC, Wei J, Zhang S, Yang B, Chen J#, Yang L#. (2019) Comparison of cytosine base editors and development of the BEable-GPS database for targeting pathogenic SNVs. Genome Biology, 20(1):218
13. Xia S, Yan L, Xu W, Agrawal AS, Algaissi A, Tseng CK, Wang Q, Du L, Tan W, Wilson IA#, Jiang S#, Yang B#, Lu L#. (2019) A pan-coronavirus fusion inhibitor targeting the HR1 domain of human coronavirus spike. Science Advances, 5(4): eaav4580
14. Chen J#, Yang B#, Yang L#. (2019) To BE or not to BE, that is the question. Nature Biotechnology, 37(5):520-522
15. Yang B#, Yang L# and Chen J#. (2019) Development and Application of Base Editors. The CRISPR Journal, 2: 91-104
16. Yan L, Meng B, Xiang J, Wilson IA# & Yang B#. (2018) Crystal structure of the post-fusion core of the Human coronavirus 229E spike protein at 1.86 Å resolution. Acta Crystallographica Section D, 74(Pt 9):841-851
17. Wang X*, Li J*, Wang Y*, Yang B*, Wei J, Wu J, Wang R, Huang X#, Chen J#, Yang L#. (2018) Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nature Biotechnology, 36(10):946-949
18. Qiang M, Dong X, Zha Z, Zuo XK, Song XL, Zhao LX, Yuan C, Huang C, Tao PD, Hu Q, Li WG, Hu WH, Li J, Nie Y, Buratto D, Zonta F, Ma PX, Yu Z, Liu LL, Zhang Y, Yang B, Xie J, Xu TL, Qu ZH#, Yang G#, Lerner RA#. (2018) Selection of an ASIC1a-blocking combinatorial antibody that protects cells from ischemic death. Proceedings of the National Academy of Sciences, 115(32): E7469-E7477
19. Li X*, Wang Y*, Liu Y*, Yang B*, Wang X, Wei J, Lu Z, Zhang Y, Wu J, Huang X#, Yang L#, Chen J#. (2018) Base editing with a Cpf1-cytidine deaminase fusion. Nature Biotechnology, 36(4):324-327
20. Lei L*, Chen H*, Xue W*, Yang B*, Hu B*, Wei J, Wang L, Cui Y, Li W, Wang J, Yan L, Shang W, Gao J, Sha J, Zhuang M, Huang X, Shen B#, Yang L#, Chen J#. (2018) APOBEC3 induces mutations during repair of CRISPR-Cas9-generated DNA breaks. Nature Structural & Molecular Biology, 25(1):45-52
21. Wang L*, Xue W*, Yan L*, Li X, Wei J, Chen M, Wu J, Yang B#, Yang L#, Chen J#. (2017) Enhanced base editing by co-expression of free uracil DNA glycosylase inhibitor. Cell Research, 27(10):1289-1292
22. Yang B#, Li X, Lei L, Chen J#. (2017) APOBEC: From mutator to editor. Journal of Genetics and Genomics, 44(9):423-437
23. Yang B, Stjepanovic G, Shen Q, Martin A, Hurley JH#. (2015) Vps4 disassembles an ESCRT-III filament by global unfolding and processive translocation. Nature Structural & Molecular Biology, 22(6):492-8
24. Hurley JH#, Yang B. (2014) Making sense of Vps4. The Journal of Molecular Biology, 426(3):503-6
25. Yang B, Zhong C, Peng Y, Lai Z, Ding J#. (2010) Molecular mechanisms of off-on switch of activities of human IDH1 by tumor-associated mutation R132H. Cell Research, 20:1188-1200 (Cover story)
26. Dong X*, Yang B*, Li Y, Zhong C, Ding J#. (2009) Molecular basis of the acceleration of the GDP-GTP exchange of human ras homolog enriched in brain by human translationally controlled tumor protein. The Journal of Biological Chemistry, 284:23754-23764
27. Dong X*, Zhou M*, Zhong C, Yang B, Shen N, Ding J#. (2009) Crystal structure of Pyrococcus horikoshiitryptophanyl-tRNA synthetase and structure-based phylogenetic analysis suggest an archaeal origin of tryptophanyl-tRNA synthetase. Nucleic Acids Research, 38:1401-1412
28. Shen N, Zhou M, Yang B, Yu Y, Dong X, Ding J#. (2008) Catalytic mechanism of the tryptophan activation reaction revealed by crystal structures of human tryptophanyl-tRNA synthetase in different enzymatic states. Nucleic Acids Research, 36:1288-1299
29. Shen N*, Guo L*, Yang B, Jin Y and Ding J#. (2006) Structure of human tryptophanyl-tRNA synthetase in complex with tRNATrp reveals the molecular basis of tRNA recognition and specificity. Nucleic Acids Research, 34:3246-3258
